Earticle

초록

영어

Background: Largemouth bass (Micropterus salmoides) is introduced species that has caused aquatic ecology activity both in vitro and in vivo were investigated for the possibility of application of the bass extract as an alternative feed ingredient. Methods: The bass oil was extracted using a 1-L supercritical extractor, while the protein was extracted from 250 g of bass dry matter, which was dissolved in 1 mL of H2O at 50℃. Both oil and protein extracts were evaluated antioxidant activities and the level of DPPH radical scavenging assay and nitric oxide (NO) production assay with lipopolysaccharide response. Oral administration of 6.6 μL/g bass protein and 5.38 μL/g bass oil conducted for investigating serological and physiological effect. Results: DPPH radical scavenging showed similar radical scavenging ability of 50 μM of ascorbic acid at 200 μg of protein and 10% of oil treatment. NO concentration was diminished by the treatment of bass oil. Oral administration of both bass oil and proteins to mice showed that the body weight increase rate of the bass oil treated group was significantly reduced by 1.55 g compared to the other groups. The number of white blood cells (WBC) was increased by 4.52 k/μL in the bass protein-treated group and 4.44 k/μL in the bass oil-treated group compared to the control group. However, the serum IgG level did not show a significant difference between the bass extract-treated groups and the control group. Conclusions: These studies demonstrate that both bass oil and proteins extracted from the bass not only provide excellent effects of antioxidant and immune activity but can also be used as functional food supplements.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Extraction and analysis of oil and protein fromMicropterus salmoides
DPPH radical scavenging activity of oil and protein
Nitric oxide assay of bass extraction and mixture
Cytotoxic analysis of bass extraction in Madin-Darbycanine kidney cells
Blood collection and analysis in mice
Enzyme-linked immunosorbent assay forImmunoglobulin G analysis
RESULTS
Oil extraction and fatty acids analysis
Soluble protein extract and taurine analysis
Cytotoxic effects and antioxidant activities oflargemouth bass extracts
Immune modulation effects of largemouth bassextracts
Effects of largemouth bass extracts on physiologicalchanges in mice
Complete blood count of mice treated with largemouthbass extracts
IgG concentration in mice treated with largemouthbass extracts
DISCUSSION
REFERENCES

키워드

저자

• Min-Gi Han [ Department of Stem Cells and Regenerative Technology, Konkuk University, Seoul 05029, Korea ]
• Ran Lee [ Department of Stem Cells and Regenerative Technology, Konkuk University, Seoul 05029, Korea ]
• Hyun Jung Park [ Department of Animal Biotechnology, Sangji University, Wonju 26339, Korea ]
• Kyung Hoon Lee [ Research Institute, Bellys Co. Ltd, Incheon 22219, Korea ]
• Hyuk Song [ Department of Stem Cells and Regenerative Technology, Konkuk University, Seoul 05029, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636