Earticle

초록

영어

Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2 , Gsr , HO-1, and catalase , were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2 , HO-1 , and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Preparation of alcoholic BSE
DPPH and ABTS assays
Cell culture and treatment
CellROX staining and flow cytometry
Preparation and culture of mouse testis fragments(MTFs)
Isolation of RNA and qPCR analysis
Western blotting
Histology and immunostaining
Statistical analysis
RESULTS
Antioxidant activity of BSE
BSE increased the expression of antioxidation-relatedgenes in LPS-induced oxidative stress
BSE inhibited mitochondrial dysfunction in cells withLPS-induced oxidative stress
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Won-Young Lee [ Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea ]
• Hyun-Woo Shim [ Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea ]
• Hyun-Jung Park [ Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636