Earticle

초록

영어

Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
DNA isolation
Material for disclosure and statistical analysis of GBLUP
Bovine satellite cell isolation
Bovine satellite cell culture and differentiation
Cell growth analysis
Immunofluorescence staining bovine satellite cell
Gene expression analysis by qRT-PCR
Statistical analysis
RESULTS
Selection of Hs-GEBV and Ls-GEBV Hanwoo by Hanwoo 50K SNP analysis bead chip and GBLUP methods
Analysis of characteristics of short-term culture of Hs-GEBV and Ls-GEBV bovine satellite cells in vitro
Analysis of characteristics of long-term culture of Hs-GEBV and Ls-GEBV bovine satellite cells in vitro
Capability of differentiation of Hs-GEBV and Ls-GEBV bovine satellite cells
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Jae Ho Han [ Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Korea ]
• Ji Suk Yu [ Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Korea ]
• Do Hyun Kim [ Department of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea ]
• Hyun Woo Choi [ Department of Agricultural Convergence Technology, Jeonbuk National University, Department of Animal Science, Jeonbuk National University, Jeonju 54896, Ko ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636