Earticle

초록

영어

Background: A breast cancer is the second leading cause of cancer death in women worldwide and among different types of breast cancers, triple-negative breast cancer (TNBC) has a poor prognosis. Methods: We investigated the potential of ginsenoside compound K (CK), an active ingredient in the bio-transformed ginsenoside, to be used as a therapeutic ingredient by examining the effects of CK on cell proliferation, apoptosis, and cancer-related gene expressions in breast cancer cells. Results: From the results of treating MCF-7, an ER and PR-positive breast cancer cells, and MDA-MB-231 (TNBC) with CK at a concentration of 0-100 μM, the half maximal inhibitory concentration (IC50) values for each cell were 52.17 μM and 29.88 μM, respectively. And also, it was confirmed that cell migration was inhibited above the IC50 concentration. In addition, fluorescence analysis of Apoptosis/Necrosis showed that CK induced apoptosis rather than necrosis of breast cancer cells. Through qPCR, it was confirmed that the expression of genes related to apoptosis and cell cycle arrest was increased in CK-treated breast cancer cells, and it acted more effectively on TNBC. However, the expression of genes related to tumor invasion and metastasis is also increased, so it is necessary to consider the timing of application of CK as a potential therapeutic anticancer compound. Conclusions: CK showed a stronger inhibitory effect in TNBC with poor prognosis but considering the high tumor invasion and metastasis-related gene expression, the timing of application of CK should be considered.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Preparation of CK
Cell culture and CK treatment
IC50 assay
Cell migration assay
Fluorescence analysis of apoptosis and necrosis
Genetic analysis using qPCR
RESULTS AND DISCUSSION
Cell viability of CK-treated breast cancer cells and IC50 concentration of CK
Cell migration inhibitory effect of CK on breast cancer cells
Apoptosis and necrosis of breast cancer cells treated with CK
Cancer-related gene expression in CK-treated breast cancer cells
CONCLUSION
REFERENCES

키워드

저자

• Seun Eui Kim [ Genuine Research, Seoul 06040, Korea, Department of Biotechnology, Hankyong National University, Ansung 17579, Korea ]
• Myoung-Hoon Lee [ Genuine Research, Seoul 06040, Korea ]
• Hye-Myoung Jang [ Genomic Informatics Center, Hankyong National University, Ansung 17579, Korea ]
• Wan-Taek Im [ Department of Biotechnology, Hankyong National University, Ansung 17579, Korea ]
• Joontaik Lee [ Department of Biotechnology, Hankyong National University, Ansung 17579, Korea ]
• Sang-Hwan Kim [ School of Animal Life Convergence Science, Hankyong National University, Ansung, 17579, Korea ]
• Gwang Joo Jeon [ Department of Biotechnology, Hankyong National University, Genomic Informatics Center, Hankyong National University, Ansung 17579, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636