Earticle

초록

영어

Background: The successful production of superior or transgenic offspring from in vitro produced embryos in cattle relies heavily on the quality of blastocyst stage embryos. In order to enhance the developmental competency of these embryos, a novel culture method was devised. Methods: This study utilized stem cell culture medium (SCM) from hESCs as a supplement within the culture medium for bovine in vitro produced embryos. To gauge the efficacy of this approach, in vitro fertilized embryos were subjected to culture in CR1aa medium enriched with one of three supplements: 0.3% BSA, 10% FBS, or 10% SCM. Results: The blastocyst development and hatching rates of one-cell zygotes cultured in CR1aa medium supplemented with SCM (23.9% and 10.2%) surpassed those cultured in CR1aa medium supplemented with BSA (9.3% and 0.0%) or FBS (3.1% and 0.0%) (p < 0.05). Furthermore, post-zygotic gene activation, cleaved embryos cultured in CR1aa medium supplemented with SCM (57.8% and 34.5%) exhibited notably higher rates (p < 0.05) compared to those cultured with BSA (12.9% and 0.0%) or FBS (45.7% and 22.5%) supplementation. Furthermore, the microinjection of SCM into the cytoplasm or pronucleus of fertilized zygotes resulted in elevated blastocyst development and hatching rates, particularly when the microinjected embryos were subsequently cultured in CR1aa medium supplemented with SCM from the 8-cell embryo stage onwards (p < 0.05), in contrast to those cultured with FBS supplementation. Conclusions: In conclusion, this study conclusively demonstrated that the incorporation of SCM into the culture medium significantly enhances the developmental progress of preimplantation embryos.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Oocyte retrieval and in vitro maturation (IVM)
In vitro fertilization and development
Preparation of SCM
Experimental designs
Statistical analyses
RESULTS
Experiment 1. In vitro development in each CR1aa medium supplemented with BSA, FBS or SCM of in vitro maturation and fertilization oocytes
Experiment 2. In vitro development in each CR1aa medium supplemented with BSA, FBS or SCM ofcleaved embryos on day 3 after in vitro fertilization of matured oocytes
Experiment 3. In vitro development in each CR1aa medium supplemented with FBS or SCM after microinjection of medium into cytoplasm of in vitro fertilized zygotes
Experiment 4. In vitro development in each CR1aa medium supplemented with FBS or SCM after microinjection of medium into pronucleus of in vitro fertilized zygotes
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Sang Jun Uhm [ Department of Animal Science, Sangji University, Wonju 26339, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636