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Original Article

The effect of gelatin-coating on embryonic stem cells as assessed by measuring Young’s modulus using an atomic force microscope

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 38 No. 3 (2023.09)바로가기
  • 페이지
    pp.121-130
  • 저자
    Hyunhee Song, Hoon Jang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A436952

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원문정보

초록

영어
Background: Coating a culture plate with molecules that aid in cell adhesion is a technique widely used to produce animal cell cultures. Extracellular matrix (ECM) is known for its efficiency in promoting adhesion, survival, and proliferation of adherent cells. Gelatin, a cost-effective type of ECM, is widely used in animal cell cultures including feeder-free embryonic stem (ES) cells. However, the optimal concentration of gelatin is a point of debate among researchers, with no studies having established the optimal gelatin concentration. Methods: In this study, we coated plastic plates with gelatin in a concentrationdependent manner and assessed Young’s modulus using atomic force microscopy (AFM) to investigate the microstructure of the surface of each plastic plate. The adhesion, proliferation, and differentiation of the ESCs were compared and analyzed revealing differences in surface microstructure dependent on coating concentration. Results: According to AFM analysis, there was a clear difference in the microstructure of the surface according to the presence or absence of the gelatin coating, and it was confirmed that there was no difference at a concentration of 0.5% or more. ES cell also confirmed the difference in cell adhesion, proliferation, and differentiation according to the presence or absence of gelatin coating, and also it showed no difference over the concentration of 0.5%. Conclusions: The optimum gelatin-coating for the maintenance and differentiation of ES cells is 0.5%, and the gelatin concentration-mediated microenvironment and ES cell signaling are closely correlated.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Materials
Young’s modulus analysis using AFM
ESC maintenance and seeding
CCK assay
ESC differentiation
qPCR analysis
Statistical analysis
RESULTS
Young’s modulus by AFM
Effect on cell attachment of the concentration of gelatin coating
The effect of gelatin coating on ESC differentiation
DISCUSSION
CONCLUSION
SUPPLEMENTARY MATERIALS
REFERENCES

키워드

atomic force microscopy embryonic stem cell gelatin-coating Young’s modulus

저자

  • Hyunhee Song [ Department of Life Science, Jeonbuk National University, Jeonju 54896, Korea ]
  • Hoon Jang [ Department of Life Science, Jeonbuk National University, Jeonju 54896, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 38 No. 3

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