Earticle

초록

영어

Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs. Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs. Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants. Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals and tissue preparation
Endometrial explant cultures
Total RNA extraction, reverse transcription-polymerasechain reaction (RT-PCR), and cloning of ILC marker cDNAs
Quantitative real-time RT-PCR
Immunohistochemistry
Statistical analysis
RESULTS
Expression of ILC markers, CD127, BCL11B, and RORC mRNAs in the endometrium during the estrous cycle and pregnancy in pigs
Expression of ILC markers, CD127, BCL11B, and RORC, in conceptuses during early pregnancy andchorioallantoic tissues during later stage of pregnancy
Localization of BLC11B and RORC protein in the endometrium during the estrous cycle and pregnancy in pigs
Effects of IFNG on CD127, BCL11B and RORC mRNA expression in endometrial tissues
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Yugyeong Cheon [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ]
• Inkyu Yoo [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ]
• Soohyung Lee [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ]
• Hakhyun Ka [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636