Earticle

초록

영어

Colorectal cancer is one of the most common types of cancer worldwide, ranking third after lung and breast cancer in terms of global prevalence. With an expected 1.93 million new cases and 935,000 deaths in 2020, it is more prevalent in males than in women. Evidence has shown that during the later stages of colon cancer, STAT1 promotes tumor progression by promoting cell survival and resistance to chemotherapy. Recent studies have shown that inhibiting STAT1 pathway leads to a reduction in tumor cell proliferation and growth, and can also promote apoptosis in colon cancer cells. One of the recent approaches in the field of drug discovery is drug repurposing. In drug repurposing approach we have virtually screened FDA database against STAT1 protein and their interactions have been studied through Molecular docking. Cross docking was performed with the top 10 compounds to be more specific with STAT1 comparing the affinity with STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. The drugs that showed higher affinity were subjected to Conceptual – Density functional theory. Besides, the Molecular dynamic simulation was also carried out for the selected leads. We also validated in-vitro against colon cancer cell lines. The results showed mainly Acetyldigitoxin has shown better binding to the target. From this study, we can predict that the drug Acetyldigitoxin has shown noticeable inhibitory efficiency against STAT1, which in turn can also lead to the reduction of tumor cell growth in colon cancer.

목차

Abstract
1. Introduction
2. Materials and methods
2.1. Target identification
2.2. Ligand preparation
2.3. Virtual screening
2.4. Molecular docking
2.5. Cross docking
2.6. Density functional theory
2.7. Molecular dynamic simulation
3. Results and conclusion
3.1. Virtual screening
3.2. Cross docking
3.3. DFT Calculation
3.4. Molecular dynamics simulation
4. Discussion
References

키워드

저자

• Veena Jaganivasan [ Department of Genetic Engineering School of Bioengineering SRM Institute of Science and Technology Kattankulathur, Tamil Nadu ]
• Dona Samuel Karen [ Department of Genetic Engineering School of Bioengineering SRM Institute of Science and Technology Kattankulathur, Tamil Nadu ]
• Bavya Chandrasekhar [ Department of Microbiology, School of Biological Science, CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]

• 간기

  계간

• pISSN

  2005-1042

• 수록기간

  2008~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 405 DDC 505