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Heloniopsis orientalis (Liliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.5 to 2.0 mg/L 2,4-D or 1.0 to 3.0 mg/L BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 1.0 mg/L BA (81%). BA triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.
목차
Abstract Introduction Materials and Methods Results and Discussion Acknowledgements References
Jong-Woo Nam [ Department of Applied Biology, Kangwon National University, Chuncheon 24341, Republic of Korea ]
Yoon-Kyung Choi [ Seedvault Center, Baekdudaegan National Arboretum, Bonghwa 36209, Republic of Korea ]
Kyeong-Mi Cho [ Seedvault Center, Baekdudaegan National Arboretum, Bonghwa 36209, Republic of Korea ]
Young-Been Kim [ Seedvault Center, Baekdudaegan National Arboretum, Bonghwa 36209, Republic of Korea ]
Sung Hwan Yim [ Department of Biology, Kyungpook National University, Research Institute for Dok-do Ulleung-do Island, Kyungpook National University, Daegu 41566, Republic of Korea ]
Kee Hwa Bae [ Seedvault Center, Baekdudaegan National Arboretum, Bonghwa 36209, Republic of Korea ]
Corresponding Author