This study evaluated the antioxidant and skin-whitening activity of fermented Lindera obtusiloba flower extracts and optimized the fermentation process. First, the antioxidant capacity was measured. In the DPPH assay, one strain increased the antioxidant capacity after 24 h of fermentation, and in the ABTS assay, three strains increased the antioxidant capacity after 24 h of fermentation. The TPC and TFC assays both revealed an increase in the extraction of phenolic compounds in three strains after 24 h of fermentation. Then, experiments using B16F10 cells were conducted to determine the whitening activity. No cytotoxicity was observed for the fermented flower extract in the concentration range of 12.5–100 μg/mL. Meanwhile, treatment with the fermented extract at 100 μg/mL resulted in a 34.7 ± 0.9% reduction in melanin production and a 34.2 ± 1.8% reduction in tyrosinase production versus the values before fermentation (both p<0.05). Thus, the results confirmed that fermentation of L. obtusiloba flowers can enhance their whitening effect.
한국어
본 연구는 생강나무(Lindera obtusiloba) 꽃 발효 추출물의 항산화능을 평가하고, 발효 최적화를 실시한 뒤, 이를 바탕으로 미백 활성 측정을 진행하였다. DPPH assay에서는 발효 24시간 후 1개의 균주가 항산화능을 증가시켰으며, ABTS assay에서는 발효 24시간 후 3개 균주가 항산화능을 증가시켰다. TPC assay에서는 발효 24시간 후 3개의 균주 샘플에서 페놀성 물질의 추출량이 증가함을 보였으며, TFC assay에서는 발효 24시간 후 샘플 중 3개의 균주 샘플에서 플라보노이드의 추출량이 증가함을 보였다. 미백 활성도를 측정하기 위해 B16F10을 이용한 세포 실험을 진행하였다. 생강나무 꽃 발효 추출물의 세포 독성은 12.5-100 μg/mL에서 나타내지 않았으 며, 생강나무 꽃 발효 추출물을 100 μg/mL에서 34.7±0.9%의 멜라닌 생성 억제 효과를 나타내었으며 34.2±1.8% 의 tyrosinase 생성 억제 효과를 나타내었으며, 발효 전과 비교하여 통계적으로 유의미한 차이를 보였다(p<0.05). 이를 바탕으로 생강나무 꽃의 발효는 미백 효과를 상승시킬 수 있음을 확인하였다.
Ever since next generation convergence technology became one of the most important industries in the nation, computing professionals have encountered a growing number of challenges. Along with scholars and colleagues in related fields, they have gathered in avariety of forums and meetings over the last few decades to share their knowledge, experiences and the outcome of their research. These exchanges have led to the founding of the International Next-generation Convergence technology (INCA) on December 1, 2015. INCA was registered as an incorporated association under the Ministry of Information and Communications. The main purpose of the organization is to improve our society by achieving the highest capability possible in next generation convergence technology.
간행물
간행물명
차세대융합기술학회논문지 [The Journal of Next-generation Convergence Technology Association]