아미노산 첨가에 따른 느타리 균사체 생장 및 laccase 활성 비교
Comparison of the growth and laccase activity of Pleurotus ostreatus mycelium after supplementation with amino acids
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In this study, we aimed to compare the mycelial growth of Pleurotus ostreatus after medium supplementation with various amino acids at different concentrations to select the optimal medium nutrient composition for mycelial growth. The mycelial growth of P. ostreatus was investigated after adding four amino acids (tryptophan, threonine, methionine, and lysine) at 0.5% or 1% to the medium.The rate of P. ostreatus mycelial growthwas faster in the potato dextrose agar (PDA) medium supplemented with threonine at 0.5% or 1% than that of the control, whereas it was inhibited by tryptophan treatment. Supplementation of sawdust mediumwith all amino acids, except tryptophan, at 0.5% did not alter the mycelial growth, compared to the controls. However, addition of any amino acid to sawdust medium at a higher concentration (1%) inhibited the mycelial growth. The laccase acitivity of P. ostreatus mycelium cultured in PDA medium was the highest when threonine was added, and the lowest when tryptophane was added, consistent with the results of the mycelial growth. Therefore, the addition of threonine, methionine, or lysine to PDA medium at a concentration of 0.5-1%was effective for increasing the mycelial growth of P. ostreatus; however, it inhibited mycelial growth insawdust medium, suggesting that the effects of amino acids dependedon the medium nutrient composition.
목차
ABSTRACT 서론 재료 및 방법 공시균주 Agar 배지 균사체 배양 톱밥 배지 균사체 배양 Laccase 활성 조사 통계 분석 결과 및 고찰 균사체 생장 조사 톱밥 배지 균사체 생장량 조사 Laccase 활성 조사 적요 REFERENCES