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Original Article

Supplementation of retinoic acid alone in MSC culture medium induced germ cell-like cell differentiation

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 38 No. 2 (2023.06)바로가기
  • 페이지
    pp.54-61
  • 저자
    Kuldeep Kumar, Kinsuk Das, Ajay Kumar, Purnima Singh, Madhusoodan A. P., Triveni Dutt, Sadhan Bag
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A432902

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원문정보

초록

영어
Background: Germ cells undergo towards male or female pathways to produce spermatozoa or oocyte respectively which is essential for sexual reproduction. Mesenchymal stem cells (MSCs) have the potential of trans-differentiation to the multiple cell lineages. Methods: Herein, rat MSCs were isolated from bone marrow and characterized by their morphological features, expression of MSC surface markers, and in vitro differentiation capability. Results: Thereafter, we induced these cells only by retinoic acid supplementation in MSC medium and, could able to show that bone marrow derived MSCs are capable to trans-differentiate into male germ cell-like cells in vitro. We characterized these cells by morphological changes, the expressions of germ cell specific markers by immunophenotyping and molecular biology tools. Further, we quantified these differentiated cells. Conclusions: This study suggests that only Retinoic acid in culture medium could induce bone marrow MSCs to differentiate germ cell-like cells in vitro . This basic method of germ cell generation might be helpful in the prospective applications of this technology.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
Isolation, culture and characterization of rat bone marrow derived MSCs
Induction for germ cell-like cells differentiation
RESULTS
Isolation and characterization of bone marrow derived rMSCs
In vitro differentiation of rMSCs into germ cell-like cells
DISCUSSION
CONCLUSION
REFERENCES

키워드

differentiation germ cell-like cells mesenchyamal stem cells retinoic acid

저자

  • Kuldeep Kumar [ Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Bareilly 243122, India ]
  • Kinsuk Das [ Department of Veterinary Physiology, West Bengal University of Animal & Fishery Sciences, Kolkata 70037, India ]
  • Ajay Kumar [ Biochemistry and Food Science Section, ICAR-Indian Veterinary Research Institute, Bareilly 243122, India ]
  • Purnima Singh [ Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Bareilly 243122, India ]
  • Madhusoodan A. P. [ Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Bareilly 243122, India ]
  • Triveni Dutt [ Division of Livestock Production and Management, ICAR-Indian Veterinary Research Institute, Bareilly 243122, India ]
  • Sadhan Bag [ Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Bareilly 243122, India ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

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