This study aimed to verify the whitening effect of Cordyceps militaris, which is distributed in several countries worldwide, including Korea, Japan, and China, and has various medical effects. To screen the efficacy of C. militaris, the inhibitory activity of tyrosinase, which was 66% at a concentration of 1 mg/mL, was measured. Thereafter, the survival rate of melanoma cells was measured, and cell experiments were conducted at a concentration of 90% or more in which C. militaris was not toxic to cells. After measuring the inhibitory effect of TRP-1, TRP-2, tyrosinase protein, and mRNA expression, which are factors influencing melanin synthesis, C. militaris was found to decrease in all factors, with an expression level that was significantly lower compared to quercetin. This confirmed that C. militaris stimulated with LED has excellent whitening activity and can be used as a functional whitening cosmetics material.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Fungal strains Submerged culture conditions Cell culture Cell viability assay Tyrosinase activity assay Melanin content assay Investigation of genes Investigation of protein expression RESULTS AND DISCUSSION Cell viability of melanoma cells induced by C. militaris mycelia Inhibition mushroom tyrosinase activity of C. militaris mycelia Effect of C. militaris mycelia on melanin content in B16F10 cells Genetic investigation Investigation of protein expression CONCLUSION Acknowledgements Data Availability Conflicts of Interest Authors Contributions REFERENCES
Si Young Ha [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
Ji Young Jung [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
Jae-Kyung Yang [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
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