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Original Article

Sperm motility and viability of experimental animals using different cryopreservatives

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 38 No. 1 (2023.02)바로가기
  • 페이지
    pp.38-46
  • 저자
    Won Yong Park, Byoung Boo Seo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A427108

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원문정보

초록

영어
In this study, an experiment was conducted in order to determine what cryopreservatives (CPVs) were more effective in supporting the motility and viability of sperm from experimental animals. The sperm of mice, rats, beagle dogs, and rabbits were frozen using different CPVs, including DMSO, TYB, and Sperm CryoProtec. The results from freezing the sperm of each laboratory animal in Sperm CryoProtec showed a high level of sperm motility and viability in sperm samples from mice, rats, and beagle dogs melted at the end of the first week. For rabbits, a high level of motility was observed in sperm thawed during the first week, whereas a high level of viability was observed in sperm thawed during the second week. The results of analysis of sperm motility and viability using different CPVs according to laboratory animals showed a significantly higher level of sperm motility (26.28%) and viability (36.20%) for mice in Sperm CryoProtec and the lowest levels of motility and viability were observed in DMSO (p < 0.05). Significantly higher levels of motility (27.94%) and viability (37.94%) were observed for rats in Sperm CryoProtec compared with TYB, which showed the lowest levels of motility and viability (p < 0.05). The study findings described above suggest that the selection of appropriate cryopreservatives is required for each experimental animal. This is because there are differences in the levels of sperm motility and viability of experimental animals depending on the CPVs that are typically used for freezing human sperm, including Sperm CryoProtec and TYB.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Sentinel animals
Culture medium
Pre-culture
Sperm collection
Freezing
Thawing
Measurement of sperm motility and viability
Statistical analysis
RESULTS
Motility and viability of sperm from experimental animals thawed at weekly intervals after freezing in Sperm CryoProtec
Motility and viability of sperm from experimental animals thawed at weekly intervals after freezing in TYB
Motility and viability of sperm from experimental animals thawed at weekly intervals after freezing in DMSO
Motility and viability of sperm from experimental animals thawed at weekly intervals after freezing in different CPVs
DISCUSSION
CONCLUSION
REFERENCES

키워드

cryopreservatives (CPVs) sperm cyroprotec sperm motility sperm viability test yolk buffer (TYB)

저자

  • Won Yong Park [ Department of Animal Science, Daegu University, Preclinical research center Daegu-Gyeongbok Medical Innovation Foundation (DGMIF), Daegu 41061, Korea ]
  • Byoung Boo Seo [ Department of Animal Science, Daegu University, Gyeongsan 38453, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 38 No. 1

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