Brassica oleracea var. italica (broccoli) is a type of cabbage that contains vitamins, minerals, and phytochemicals. Consequently, it is used as a potential nutraceutical source for improving human health by reducing oxidative stress and inflammatory responses. Here, the effects of broccoli sprout extract (BSE) on the inflammatory response were investigated through lipopolysaccharide (LPS)- induced inflammatory mouse models. First, we found that the BSE obviously reduce NO production in RAW 264.7 cells in response to LPS stimulation in in vitro study. Pretreatment with BSE administration improved sperm motility and testicular cell survivability in LPS-induced endotoxemic mice. Additionally, BSE treatment decreased the levels of the pro-inflammatory cytokines TNF-a, IL-1β, and IL-6, and COX-2 in testis of LPS-induced endotoxemic mice models. In conclusion, BSE could be a potential nutraceutical for preventing the excessive immune related infertility.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Preparation of BSE and HPLC Cell culture and cell viability assay Nitrate scavenging ability assay Animals and treatment Isolation of RNA and qPCR analysis Western blot studies Histology and immunostaining Sperm motility Statistical analysis RESULTS HPLC analysis of BSE Effect of BSE on cell viability RAW 364.7 macrophage and NO production Effects of BSE on LPS-induced inflammation on the testis BSE suppressed Pro-inflammatory response in testis of LPS-induced inflammation model. DISCUSSION CONCLUSION REFERENCES