Earticle

초록

영어

The purpose of this study was to determine whether combined inter-vention of treadmill exercise and bone marrow stromal cell (BMSC) transplantation would affect the expression of neurotrophic factors in the sciatic nerve injury (SNI) and neuropathic pain-related cascades in ipsilateral lumbar 4-5 dorsal root ganglion (DRG) during the early or late stage of sciatic nerve regeneration. The rats were randomly divided into the normal control group (CONT, n=6), sedentary group (SS, n=24), exercise group (SE, n=24), BMSC transplantation group (SB, n=24), BMSC transplantation+exercise group (SBE, n=24) 1, 2, 3, and 5 weeks after SNI. Single dose of 5×106 harvested BMSC was injected into the injury area sing by a 30 gauge needle. Treadmill exercise was performed at a speed of 8 m/min for 30 min once a day. Tropomyosin-receptor ki-nase B, brain-derived neurotrophic factor and ciliary neurotrophic fac-tor were significantly upregulated in the SE and SBE groups at 1- and 2-week postinjury than those in the CONT and SS groups, and SB and SBE groups continuously kept up proinflammatory cytokines until the late stage of regeneration. Nuclear factor kappa-light-chain-enhancer of activated B cells, interleukin and tumor necrosis factor alpha in ipsi-lateral DRG were progressively decreased by exercise alone application and/or BMSC transplantation at early and late stage of regeneration. Present results provide reliable information that combined intervention of treadmill exercise and BMSC transplantation might be one of the ef-fective treatment strategies for recovering sciatic nerve injury-induced neuropathic pain over time.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
Sciatic nerve injury
Treadmill exercise protocols
BMSC culture and transplantation
Western blot analysis
Statistical analysis
RESULTS
Neurotrophic factors at early stages of sciatic nerve regeneration
Neurotrophic factors at late stages of sciatic nerve regeneration
Pain-related molecules in DRG at early stages of regeneration
Pain-related molecules in DRG at late stages of regeneration
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Joo-In Yu [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Yeong-Hyun Cho [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Young-Pyo Kim [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613