Earticle

초록

영어

We aimed to explore natural products exhibiting anticancer activities against pancreatic cancer and identify the molecular mechanisms of drugs that target microenvironmental stress in pancreatic cancer cells. After establishing a screening system using GRP78 as a molecular target under microenvironmental stress, 105 different natural products were selected, and their extracts were used to treat pancreatic cancer cells. Morphological assays confirmed the anticancer activity of three natural products (Allii Tuberosi Semen, Bombycis Faecula, and Piperis Nigri Fructus). Colony formation assays performed after treatment with each of the three products showed that Piperis Nigri Fructus exhibited the highest anticancer activity; its anticancer activity was further confirmed by Hoechst 33342 staining. Finally, western blot analysis confirmedthe anticancer activity of Piperis Nigri Fructus extracts in PANC-1 cells by suppressing GRP78 molecular targeting. These results explain the cellular mechanisms of natural products with anticancer activity and confirm the anticancer activity of Piperis Nigri Fructus. These findings will be fundamental to the development of tools to identify anticancer agents in cells under microenvironmental stress as well as novel anticancer drugs.

목차

ABSTRACT
1. INTRODUCTION
1.1. Motive
1.2. Purpose
2. THEORETICAL BACKGROUND
2.1. Pancreatic structure and function
2.2. Treatment of pancreatic cancer
2.3. Mechanism underlying microenvironmental stress within human cancer cells
2.4. Cellular changes in cancer cells under microenvironmental stress
2.5. Mechanism underlying the molecular targeting of microenvironmental stress in pancreatic cancer cells
2.6. Establishing a screening system for molecular targeting of microenvironmental stress
3. MATERIALS AND METHODS
3.1. Study materials
3.2. Equipment
3.3. Study methods
4. RESULTS
4.1. Cytotoxicity for natural products with anticancer activity
4.2. Quantitative assessment of GRP78 expression using western blot in cells treated with Piperis Nigri Fructus extract
5. CONCLUSION AND DISCUSSION
6. ACKNOWLEDGEMENT
REFERENCES

키워드

저자

• Minju Ku [ Changwon Science High School, 30 Pyeongsan-ro 159beon-gil, Seosang-dong, Uichang-gu, Changwon, Republic of Kor ]
• Seunghu Byeon [ Changwon Science High School, 30 Pyeongsan-ro 159beon-gil, Seosang-dong, Uichang-gu, Changwon, Republic of Kor ]
• Suhyun Hwang [ Changwon Science High School, 30 Pyeongsan-ro 159beon-gil, Seosang-dong, Uichang-gu, Changwon, Republic of Kor ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  APEC Youth Scientist Journal [APEC 청소년 과학학술지]

• 간기

  반년간

• pISSN

  2005-5625

• 수록기간

  2009~2022

• 십진분류

  KDC 405 DDC 505