Earticle

초록

영어

The demand for novel strains has been rising in the domestic market to increase the production of sclerotia from Wolfiporia hoelen. To improve strain breeding efficiency, we investigated whether single-nucleotide polymorphisms (SNPs) in the RNA polymerase II subunit (RPB2) gene, which may be linked to the mating type locus, are useful for distinguishing monokaryons from dikaryons in Korean W. hoelen strains. We designed a specific primer set to efficiently amplify a region of RPB2 using PCR with the genomic DNA of 12 cultivated strains and 31 wild strains of W. hoelen collected from Korea. Nucleotide sequences of the PCR-amplified RPB2 genes were determined and analyzed for the presence of SNPs among the 43 W. hoelen strains. Previously reported SNP loci were detected in the RPB2 gene of all W. hoelen strains tested. However, these previously reported SNP loci could not be applied to differentiate monokaryons from dikaryons in approximately one-third of Korean wild strains with homozygous genotypes. Three additional SNPs in the RPB2 gene, which may improve the ability to distinguish monokaryons from dikaryons, were identified by searching through the multiple sequence alignments of the 43 W. hoelen strains. The applicability of these three novel SNPs, together with the previously known SNPs, in the RPB2 gene to W. hoelen strain breeding was verified by examining the hybrid strains and their parental strains.

목차

ABSTRACT
서론
재료 및 방법
균주 및 균주 접종
자실체 형성 및 단포자 분리
DNA 추출 및 PCR
결과 및 고찰
국내 복령 균주의 RPB2 유전자 내 SNP 분석
RPB2 유전자 내 SNP의 육종 활용성 평가
적요
감사의 글
REFERENCES

키워드

저자

• 김수연 [ Su Yeon Kim | 국립산림과학원 산림생명자원연구부 산림미생물연구과, 단국대학교 과학기술대학 미생물학과 ]
• 박미정 [ Mi-Jeong Park | 국립산림과학원 산림생명자원연구부 산림미생물연구과 ]
• 김성환 [ Seong Hwan Kim | 단국대학교 과학기술대학 미생물학과 ]
• 가강현 [ Kang-Hyeon Ka | 국립산림과학원 산림생명자원연구부 산림미생물연구과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635