Earticle

초록

영어

The cellular communication network factor (CCN) family proteins regulate many biological events such as angiogenesis, tumor growth, placentation, implantation, and embryogenesis. The expression and function of CCN1, CCN2, and CCN3 at the maternal-conceptus interface are established in humans and rodents, but little is known about the role of CCN4 to CCN6 in the reproductive organs in any other species. Several studies in transcriptome analysis in pigs have shown that the expression of CCN4 and CCN6 increases in the endometrium during early pregnancy. However, their expression, regulation, and function in the endometrium throughout the estrous cycle and pregnancy have not been fully understood in pigs. Thus, we determined the expression, localization, and regulation of CCN4 and CCN6 during the estrous cycle and at the maternal-conceptus interface in pigs. We found that the levels of CCN4, but not CCN6, changed during the estrous cycle. The levels of CCN4 were greater during mid- to late pregnancy than in the early stage, and the levels of CCN6 were greatest on Day 15 of pregnancy. CCN4 and CCN6 were detected in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. CCN4 mRNA was mainly localized to epithelial cells, CCN6 mRNAs to epithelial and stromal cells in the endometrium. In endometrial explant cultures, CCN4 expression was increased by progesterone, and CCN6 expression by interferon-γ. These results suggest that CCN4 and CCN6 may play roles in the establishment and maintenance of pregnancy by regulating the endometrial epithelial cell functions in pigs.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals and tissue preparation
Explant cultures
Porcine uterine endometrial epithelial cell culture
Total RNA extraction and RT-PCR for CCN4 and CCN6 cDNAs
Quantitative real-time RT-PCR
In situ hybridization
Statistical analysis
RESULTS
Expression of CCN4 and CCN6 mRNAs in the endometrium during the estrous cycle and pregnancy
Expression of CCN4 and CCN6 mRNAs in conceptus tissues during early pregnancy and chorioallantoic tissues during later stage pregnancy
Effects of E2, P4, and IFNG on the expression of CCN4 and CCN6 mRNAs in endometrial tissues
Effects of IL10 on CCN4 and CCN6 mRNA expression in pUE cells
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Inkyu Yoo [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ]
• Soohyung Lee [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ]
• Yugyeong Cheon [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ]
• Hakhyun Ka [ Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636