Earticle

초록

영어

Protein and peptide candidates are screened to apply therapeutic application as a drug. Ensuring that these candidates are delivered and maximized effectiveness is still challenging and a variety of studies are ongoing. As drug delivery system vehicles, cell-penetrating peptide (CPP) can deliver various kinds of cargo into the cell cytosol. In a previous study, we developed Ara27 CPP, which are a zinc knuckle family protein of Arabidopsis, and confirmed internalization in human dermal fibroblasts and human dental pulp stem cells at low concentration with short time treatment condition without any toxicity. Ara27, an amphipathic CPP, could be modified and utilized in the biomedical field excluding the risk of toxicity. Therefore, we would like to confirm the non-toxic induced penetrating ability of Ara27 in various cell lines. The purpose of this study was to screen the cell internalization ability of Ara27 in various cell lines and to confirm Ara27 as a promising core CPP structure. First, Ara27 was screened to confirm non-toxicity concentration. Then, fluorescence-labeled Ara27 was treated on human normal cell lines, cancer cell lines and animal cell lines to identify the cellular internalization of Ara27. Ara27 was well intracellular localized in all cell lines and the intensity of fluorescence was remarkably increased in time pass manner. These results indicate that Ara27 has the potential as a core structure for applications in various drug delivery systems.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Culture of multiple cell lines
Peptide synthesis and preparation
Cell viability assay
Immunofluorescence staining
RESULTS
Cell viability of Ara27 in HDF and HaCaT
nternalization of Ara27 in HDF and HaCaT
Internalization of Ara27 in A549 and H460
Internalization of Ara27 in BDF and PFF
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Minseo Kim [ Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University School of Dentistry, Seoul 08826, Korea ]
• Sangkyu Park [ Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University, Biomedical Research Institute, NeoRegen Biotech Co., Ltd., Suwon 16614, Korea ]
• Jeongmin Seo [ Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University, Biomedical Research Institute, NeoRegen Biotech Co., Ltd., Suwon 16614, Korea ] Corresponding Author
• Sangho Roh [ Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University School of Dentistry, Seoul 08826, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636