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Original Article

The antioxidant capacity of Mito-TEMPO improves the preimplantation development and viability of vitrified-warmed blastocysts through the stabilization of F-actin morphological aspects in bovine embryos

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 37 No. 4 (2022.12)바로가기
  • 페이지
    pp.231-238
  • 저자
    Jae-Hoon Jeong, Hyo-Jin Park, Seul-Gi Yang, Deog-Bon Koo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A423439

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원문정보

초록

영어
Reactive oxygen species (ROS) production and F-actin cytoskeleton dynamics play important roles in the survival rate of blastocysts after the vitrifiedwarming process. However, the protective effects of Mito-TEMPO against cryo-injury and viability through F-actin aggregation and mitochondrial-specific ROS production in vitrificated-warmed bovine embryos have not been investigated. The aims of the present study were to: (1) determine the effects of Mito-TEMPO on embryonic developmental competence and quality by F-actin stabilization during in vitro culturing (IVC), and (2) confirm the effects of Mito-TEMPO through F-actin structure on the cryotolerance of vitrification-warming in Mito-TEMPO exposed in vitro production (IVP) of bovine blastocysts. Bovine zygotes were cultured with 0.1 μM Mito-TEMPO treatment for 2 days of IVC. Mito-TEMPO (0.1 μM) exposed bovine embryos slightly improved in blastocyst developmental rates compared to the non-treated group. Moreover, the viability of vitrified-warmed blastocysts from Mito-TEMPO treated embryos significantly increased (p < 0.05, non-treated group: 66.7 ± 3.2% vs Mito-TEMPO treated group: 79.2 ± 5.9%; re-expanded at 24 hours). Mito-TEMPO exposed embryos strengthened the F-actin structure and arrangement in the blastocyst after vitrification-warming. Furthermore, the addition of Mito-TEMPO into the IVC medium enhanced embryonic survival and quality through F-actin stabilization after the vitrification-warming procedure. Overall, our results suggest that supplementing the culture with 0.1 μM Mito- TEMPO improves the embryonic quality and cryo-survival of IVP bovine blastocysts.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
In vitro production (IVP) of bovine embryos
Vitrification and warming procedure
Filamentous actin (F-actin) staining
Statistical analysis
RESULTS
Effect of Mito-TEMPO on the developmental rate of bovine embryos in vitro
The positive effect of Mito-TEMPO on survival and cytoskeletal structure of vitrified-warmed bovine blastocysts
DISCUSSION
CONCLUSION
REFERENCES

키워드

bovine blastocyst cryopreservation F-actin in vitro culture (IVC) Mito-TEMPO

저자

  • Jae-Hoon Jeong [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ]
  • Hyo-Jin Park [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ]
  • Seul-Gi Yang [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ]
  • Deog-Bon Koo [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 37 No. 4

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