Earticle

초록

영어

High levels of proinflammatory cytokines have been observed in obese pregnancies. Obesity during pregnancy may increase the risk of various pregnancyrelated complications, with pathogenesis resulting from excessive inflammation. Palmitic acid (PA) is a saturated fatty acid that circulates in high levels in obese women. In our previous study, we found that PA inhibited the proliferation of trophoblasts developing into the placenta, induced apoptosis, and regulated the number of cleaved halves derived from transfer RNAs (tRNAs). However, it is not known how the expression of tRNA-derived stress-induced RNAs (tiRNAs) changes in response to PA treatment at concentrations that induce inflammation in human trophoblasts. We selected concentrations that did not affect cell viability after dose-dependent treatment of HTR8/SVneo cells, a human trophoblast cell line. PA (200 μM) did not affect the expression of apoptotic proteins in HTR8/SVneo cells. PA significantly increased the expression of inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-8 , and tumor necrosis factor (TNF)-α . In addition, 200 μM PA significantly increased the expression of tiRNAs compared to 800 μM PA treatment. These results suggest that PA impairs placental development during early pregnancy by inducing an inflammatory response in human trophoblasts. In addition, this study provides a basis for further research on the association between PA-induced inflammation and tiRNA generation.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
Cell culture
MTT assay
Western blot analysis
Annexin V and propidium iodide staining
Quantitative PCR
Statistics
RESULTS
Selection of a concentration of PA that does not affect the viability of trophoblasts
PA increases the expression of inflammatory cytokines in trophoblasts
PA regulates the expression of tiRNAs in trophoblasts
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Changwon Yang [ Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea ]
• Garam An [ Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea ]
• Jisoo Song [ Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea ]
• Gwonhwa Song [ Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea ] Corresponding Author
• Whasun Lim [ Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636