Earticle

초록

영어

Polydeoxyribonucleotide (PDRN), which is adenosine A2A receptor ago-nist, facilitates healing and inhibits inflammation and apoptosis. The ef-fect of PDRN on alcoholic liver injury (ALI) was evaluated focusing on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway. The mice were given daily oral administration of 50% ethanol at a dose of 4 g/kg during 8 weeks. After 4 weeks of alcohol intake, 200 μL of normal saline containing 8-mg/kg PDRN was intraperitoneally administered 3 times a week for 4 weeks. To determine whether the action of PDRN occurs through the adenosine A2A receptor, 8-mg/kg 3,7-dimethyl-1-propargylxanthine (DMPX) with PDRN was treated. The concentration of aspartate aminotransferase (AST) and alanine amino-transferase (ALT) was detected. For liver histopathological score, he-matoxylin and eosin staining was conducted. Enzyme-linked immuno-assay was used to measure cyclic adenosine-3′,5′-monophosphate (cAMP) concentration. PI3K and Akt expression was determined using Western blot analysis. In the results, PDRN treatment suppressed AST and ALT level in serum and liver tissue, and improved damaged liver tis-sue and decreased histological score. PDRN application inhibited the expression of phosphorylated PI3K/Akt signaling pathway. The increas-ing effect of PDRN on cAMP level ats as a mechanism for ALI treatment. Co-treatment of DMPX with PDRN did not reduce apoptosis, causing no improvement in liver function. As a result of this experiment, PDRN has the potential to be selected as a therapeutic agent for ALI.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals
Inducing ALI
Blood sampling and tissue preparation
H&E staining
Measurement of AST and ALT level
Measurement of cAMP concentrations
Western blotting
Data analysis
RESULTS
Concentration of AST and ALT
Histological score
cAMP concentration
PI3K/Akt signaling expression
DISCUSSION
REFERENCES

키워드

저자

• Young-A Cho [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jun-Jang Jin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Lakkyong Hwang [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Sang-Hoon Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Department of Sport and Health Sciences, College of Art and Culture, Sangmyung University, Seoul, Korea ]
• Jung Won Jeon [ Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Myoung Joo Yang [ Department of Sports Science, Hongik University, Sejong, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613