As the seizures of cannabis have risen since 2018, the presumptive identification of THC has been studied to determine the presence of THC in seized cannabis using color tests. Because the color tests are simple to perform, inexpensive and give results that can be viewed by the naked eyes, four different color tests were examined in this study. Duquenois- Levine reagent, Fast blue B salt, Fast blue BB salt and 4-Aminophenol were compared to detect THC in the Cannabis specimens. The seized cannabis flowers were used as specimens and four reagents of Duquenois-Levine, Fast blue B salt, Fast blue BB salt, 4-Aminophenol were prepared according to the guidelines of the UNODC. GC-MS was utilized to measure the LODs of THC for the color tests applied. Duquenois-Levine, Fast blue B salt, Fast blue BB salt and 4-Aminophenol showed distinctive color changes in the presence of cannabis. By Duquenois-Levine, the color changed to blue-violet, Fast blue B salt to purple-red color, Fast blue BB salt to reddish orange color and 4-Aminophenol to blue. The LODs of THC by the four reagents were as follow: Duquenois-Levine and 4-Aminophenol showed 2 μg, Fast blue B salt was 0.6 μg and Fast blue BB salt was 0.8 μg. It was interesting to note that the blue color by 4-Aminophenol indicated the presence of THC, while pink color indicated the presence of CBD. In conclusion, Fast blue B salt was the most applicable color test to determine the presence of cannabis with the lowest LOD of THC.
목차
Abstract Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 재료 2. 시약 제조 및 실험 방법 Ⅲ. 결과 및 고찰 1. 건조된 대마 화서부위의 color test 결과 2. GC-MS 에 의한 시료 중 THC 함량 3. Color test 의 검출한계(LOD) 측정 Ⅳ. 결론 Ⅴ. 참고문헌
법과학 분야는 사회정의 구현에 있어 크나큰 가치가 있음에도 불구하고 우리나라에서는 이 분야에 대한 인식이 미흡하여 선진 외국에 비해 침체되어 있는 실정이다. 이에 우리나라에서도 법과학 분야와 관련 있는 학계, 연구기관, 수사기관 등 유관 단체들로 구성된 한국 법과학회를 창립하여 이 분야를 활성화 시켜 과학수사를 한층 더 발전시키기 위함을 목적으로 한다.