Earticle

초록

영어

Oxygen is an essential element for animal life. Although deaths due to lack of oxygen occur frequently under various indoor conditions, verifying hypoxia as the cause of mortality has remained elusive. Also, the biomarkers indicating the hypoxia-induced death have not yet been clearly identified. In this study, we screened potential metabolites of which levels in animal blood can be differentiated under hypoxic conditions. Metabolites of post-mortem bloods exposed to various low-oxygen levels (3%, 5%, 8% and 12%) were compared with those from mice ambient air. GC/MS analysis showed increase in levels of lactic acid, succinic acid, uric acid and uracil in post-mortem blood from mice exposed to moderately low oxygen level (12%). Whereas samples from mice in much lower oxygen level (3% and 5%) did not show significant differences in metabolites levels. Importantly, the increase rate of these metabolites appeared to be proportional to survival time after hypoxia, suggesting that there are hypoxia-inducible cell responses including the avoidance of oxygen-dependent energetic metabolism. The results suggest that both oxygen concentration and duration exposed to hypoxia condition determine cellular metabolic pathways to increase their consequent metabolites in animal fluids, which may be used as biomarkers for tracking hypoxia.

목차

Abstract
Ⅰ. 서론
Ⅱ. 실험방법
1. 실험동물과 시약 및 장비
2. 시료 전처리
Ⅲ. 결과 및 고찰
Ⅳ. 결론
Ⅴ. 참고문헌

키워드

저자

• 정용애 [ Yong Ae Jeong | 질병관리청 건강위해대응과 연구원 ]
• 김춘삼 [ Choon Sam Kim | 제일메디칼센터 팀장 ]
• 이준배 [ Joon-Bae Lee | 대구과학수사연구소 독성화학과 감정관 ]
• 김준모 [ Junmo Kim | 대구과학수사연구소 법의학과 법의관 ]
• 방일수 [ Iel-Soo Bang | 조선대학교 치과대학 구강미생물학 및 면역학 교실 교수 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국법과학회지 [Korean Journal of Forensic Science]

• 간기

  반년간

• pISSN

  1598-0715

• 수록기간

  2000~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 367 DDC 347