Earticle

초록

영어

The purpose of this study was to determine whether the timing of tread-mill exercise application can control expression levels of neuropathic pain- and regeneration-related proteins in the ipsilateral lumbar 4 (L4) to 6 (L6) dorsal root ganglion cells (DRG) after sciatic nerve injury (SNI). The experimental rats were randomly divided into five groups: the nor-mal control, SNI+sedentary (IS), exercise+SNI (EI), SNI+exercise (IE), exercise+SNI+exercise (EIE) groups. The rats in exercise groups per-formed treadmill exercise at a speed of 8 m/min for 30 min once a day during 14 days before and/or after SNI. For investigating the expression of specific neuropathic pain and regeneration-related proteins in DRG, we prepared L4 to L6 DRG in the ipsilateral side. In the quantitative anal-ysis, growth associated protein 43 (GAP-43) and brain-derived neuro-trophic factor levels were further increased in the ipsilateral DRG at all treadmill exercise groups than those in IS group. In the histological findings, GAP-43 was qualitatively increased IE and EIE groups than IS group at DRG. Wnt3a and β-catenin were dramatically downregulated in EIE and IE groups than IS groups. In addition, nuclear factor kappa-light-chain-enhancer of activated B cells and tumor necrosis factor-α were significantly decreased in IE and EIE groups than IS group in the ipsilateral DRG. Our findings suggested novel information that regular low-intensity exercise before and/or after SNI might be a therapeutic and preventive approaches for relieving neuropathic pain and improv-ing axonal elongation after peripheral nerve injury.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
Sciatic nerve injury
Treadmill exercise protocols
Western blot analysis
Immunofluorescence staining
Statistical analysis
RESULTS
The timing point of exercise application regulated expression levels of GAP-43 and BDNF
Expression of Wnt3a and β-catenin at the timing point of exercise application
Expression of NF-κB and TNF-α at the timing point of exercise application
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Yeong-Hyun Cho [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613