Earticle

초록

영어

Lipopolysaccharide (LPS) is an endotoxin factor present in the cell wall of Gram-negative bacteria and induces various immune responses to infection. Recent studies have reported that LPS induces cellular stress in various cells including oocytes and embryos. Melatonin (N-acetyl-5-methoxytryptamine) is a regulatory hormone of circadian rhythm and a powerful antioxidant. It has been known that melatonin has an effective function in scavenging oxygen free radicals and has been used as an antioxidant to reduce the cytotoxic effects induced by LPS. However, the effect of melatonin on LPS treated early embryonic development has not yet been confirmed. In this study, we cultured mouse embryos in medium supplemented with LPS or/and melatonin up to the blastocyst stage in vitro and then evaluated the developmental rate. As a result of the LPS-treatment, the rate of blastocyst development was significantly reduced compared to the control group in all the LPS groups. Next, in the melatonin only treated group, there was no statistical difference in embryonic development and no toxic effects were observed. And then we found that the treatment of melatonin improved the rates of compaction and blastocyst development of LPS-treated embryos. In addition, we showed that melatonin treatment decreased ROS levels compared to the LPS only treated group. In conclusion, we demonstrated the protective effect of melatonin on the embryonic developmental rate reduced by LPS. These results suggest a direction to improve reproduction loss that may occur due to LPS exposure and bacterial infection through the using of melatonin during in vitro culture.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animal care and experimentation
Hormone treatment and oocytes preparation
Sperm preparation
Culture and observation of embryos
Treatment of LPS and melatonin
Statistical analysis
RESULTS
Effects of LPS on early mouse embryo development
Effect of melatonin on early mouse embryo developmental rate
Effect of melatonin on LPS-treated early mouse embryos
Effect of LPS or/and melatonin on levels of ROS in mouse embryo
DISSCUSSION
REFERENCES

키워드

저자

• Haeun Park [ Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, Korea ]
• Hoon Jang [ Department of Life Sciences, Jeonbuk National University, Jeonju 54896, Korea ]
• Youngsok Choi [ Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Institute of Advanced Regenerative Biotechnology, Konkuk University, Seoul 05029, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636