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Comparison of Cryoprotectants and Cryopreservation Protocols for Eleutherococcus senticosus via Somatic Embryogenesis

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science KCI 등재 바로가기
  • 통권
    제38권 제3호 (2022.09)바로가기
  • 페이지
    pp.152-158
  • 저자
    Chang Ho Ahn, Jung Won Shin, Ha Na Lee, Hyun Won Yoon, Jeong Min Seo, Yeoung Ryul Kim, Saeng Geul Baek, Jae Ik Nam, Yong Eui Choi
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A417780

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원문정보

초록

영어
A cryopreservation is an essential tool for preservation of germplasm. In this study, the possibility for cryopreservation of embryogenic cells of Siberian ginseng (Eleutherococcus senticosus) in liquid nitrogen (-196°C) was evaluated. The effects of glycerol and dimethyl sulfoxide (DMSO) at different concentrations (5%, 10% and 20%) as cryoprotectants on regrowth of cryopreserved E. senticosus embryogenic cells were tested. There was significant effect of cryoprotectants on regrowth of embryogenic cells (p=0.0019). The highest and lowest fresh mass gain were achieved when embryogenic cells were frozen with 10% DMSO and 5% glycerol (138.2±5.9 and 61.3±14.6, respectively). The effect of the cryoprotectants on the frequency embryo germination was tested. There was no significant difference between glycerol and DMSO (p=0.846). Three different concentrations of cryoprotectants did not significantly affect the frequency embryo germination (p=0.534). Finally, the genetic fidelity of the plantlets regenerated from non-cryopreserved and cryopreserved embryogenic cells was tested by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis. RAPD and ISSR analysises showed that there was no genetic variation among regenerants.

목차

Abstract
Introduction
Materials and Methods
Plant material and embryogenic callus induction
Cryopreservation procedure (Freezing, thawing and recovery)
Somatic embryos production and germination
DNA extraction and RAPD and ISSR analysis
Statistical analysis
Results and Discussion
The effect of cryoprotectants on regrowth of embryogenic cells and somatic embryo germination
Genetic fidelity by RAPD and ISSR markers
References

키워드

Siberian ginseng cryopreservation cryoprotectant genetic fidelity somatic embryogenesis

저자

  • Chang Ho Ahn [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Jung Won Shin [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Ha Na Lee [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Hyun Won Yoon [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Jeong Min Seo [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Yeoung Ryul Kim [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Saeng Geul Baek [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Jae Ik Nam [ Division of Garden Material Research, Korea Institute of Arboretum Management, Sejong 30106, Republic of Korea ]
  • Yong Eui Choi [ Division of Forest Sciences, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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