Earticle

초록

영어

Many plant polysaccharide have immunomodulatory and anti-tumour activities. In the present study, water-soluble polysaccharides were isolated from an edible plant seed mainly by hot water extraction, 75% ethanol precipitation, and DEAE-cellulose column chromatography and tentatively named as PSP-F1. The PSP-F1 was shown to be a mixture of three major polysaccharides with molecular mass of approximately 1,200, 640, and 300 kDa, respectively, as estimated by size-exclusion HPLC analysis. The HPAEC-PAD analysis for neutral sugar composition and showed that PSP-F1 consists of arabinose (Ara) and xylose (Xyl), as the major neutral sugars, and other minor neutral sugars such as rhamnose (Rha), galactose (Gal), and glucose (Glc). HPLC analysis for uronic acids showed that galacturonic acid (GalA) presents as the major uronic acid and glucuronic acid (GlcA) was the minor one. Together with the neutral monosaccharides and uronic acids composition, the FT-IR and NMR analysis suggested that PSP-F1 appears to be a mixture of pectin type and heteroxylan polysaccharides. The results showed that the PSP-F1 (0-100 μg/ml) significantly induced the secretion of pro-inflammatory cytokines TNF-α and IL-6 and upregulation of MAPKs (ERK, JNK and p38) phosphorylation. The secretion of various pro-inflammatory cytokines from macrophages is mainly caused by MAPK cascade activation. These results demonstrated that the PSP-F1 isolated from a plant seed is a mixture of pectin type and heteroxylan polysaccharides, which are typical for plant cell wall polysaccharides, and that it effectively stimulates macrophages to secrete pro-inflammatory cytokines, such as TNF-α and IL-6, by upregulation of MAPKs phosphorylation. The results of this study suggests that PSP-F1 may be potential immunostimulator for health benefits and as a pharmaceutical component.

저자

• Sarang Cho [ Department of Biotechnology, Graduate School, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
• Peter Capek [ Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovakia ]
• Roman Bleha [ Department of Carbohydrates and Cereals, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic ]
• Andrej Sinica [ Department of Carbohydrates and Cereals, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic ]
• Jung Sik Lim [ Department of Biotechnology, Graduate School, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
• Jin Ree [ Department of Biotechnology, Graduate School, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
• Yong Il Park [ Department of Biotechnology, Graduate School, The Catholic University of Korea, Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614