Saccharapolyspora spinosa is the producer of the environmentally friendly insecticide spinosad, which is a combination of spinosyn A and D. spinosyn is a tetracyclic polyketide aglycone to which is attached a neutral saccharide substituent (2,3,4-O-trimethyla-L-rhamnosyl) on the C-9 hydroxyl group and an aminosugar moiety (β D-forosaminyl) on the C-17 hydroxyl group). Both the rhamnose and forosamine moieties are essential for the insecticidal activity of spinosyns. We construct a vector; pIBR-SPN, cloned the sugar genes to it and transformed to Sa. spinosa. The best strain Sa. spinosa MUV pIBR-SPN FR produced 1,896.77 mg/L of spinosad, 17 folds higher than parent strain.
Adzemye Fovennso Bridget [ Department of Life Science and Biochemical Engineering, Sun Moon University ]
Rubin Thapa Magar [ Department of Life Science and Biochemical Engineering, Sun Moon University ]
Jae Kyung Sohng [ Department of Life Science and Biochemical Engineering, Sun Moon University, Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University ]
Corresponding Author
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.