Understanding glycan-protein interactions provide valuable information on biological processes in living organisms. However, many roles of glycans in biology are still veiled. The primary reason might be the limitation of access to a glycan library with diverse structures, which hampers extensive studies on molecular interactions of glycans. Here, a functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and apply glycan-involved applications simultaneously. GM1 pentasaccharide and Globo H hexasaccharide-related complex glycans were successfully synthesized on a chip through consecutive glycosyltransferase reactions along with small amounts of enzymes and donors, without any additional processes. Biosynthesized complex glycans were demonstrated to provide information on glycan-related interactions. Thus, the proposed on-chip glycan biosynthesis system can provide a new direction toward obtaining complex glycan sources and complex glycan-involved applications such as glycan-protein interaction analysis and glycan biomarker screening.
저자
Chang Sup Kim [ School of Chemistry and Biochemistry, Yeungnam University ]
Corresponding Author
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.