High-throughput single-cell RNA sequencing (scRNA-seq) technology provide opportunities to identify the entire cellular and molecular difference based on cell-to-cell heterogeneity in gene expression. This study demonstrates the feasibility and utility of scRNA-seq in transgenic Nicotiana tabacum (N. Tabacum) leaves expressing anti-rabies virus mAb(P) SO57 and provides generation gene expression map of the plant leaf at single-cell resolution. Protoplasts isolated from the N. Tabacum leaves at non-transgenic and transgenic plants, and the transcript levels of > 20,000 genes were analyzed in 24,000 single cells from non-transgenic and transgenic plants. Total of 9 putative cell clusters and the cluster-specific marker genes were identified. We performed cell clustering analysis to determine which cell clusters were mainly synthesis exogenous genes. In conclusion, the high-resolution single cell atlas of gene expression shows the developmental landscape of N. tabacum leaf and exogenous gene expression patterns in each clusters.
저자
Seung-Won Lee [ Department of Medicine, College of Medicine, Chung-Ang University, Seoul, Korea ]
Do-Sun Kim [ Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), Wanju-gun, Jeollabuk-do, Korea ]
Ye-Rin Lee [ Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), Wanju-gun, Jeollabuk-do, Korea ]
Kisung Ko [ Department of Medicine, College of Medicine, Chung-Ang University, Seoul, Korea ]
Corresponding Author
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.