Earticle

현재 위치 Home

Short Communication

Cryopreservation of Siberian tiger (Panthera tigris altaica ) epididymal spermatozoa : pilot study of post-thaw sperm characteristics

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 37 No. 2 (2022.06)바로가기
  • 페이지
    pp.130-135
  • 저자
    Saddah Ibrahim, Nabeel Abdelbagi Hamad Talha, Jeongho Kim, Yubeol Jeon, Iljeoung Yu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A414534

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

원문정보

초록

영어
Epididymal sperm cryopreservation provides a potential method for preserving genetic material from males of endangered species. This pilot study was conducted to develop a freezing method for tiger epididymal sperm. We evaluated post-thaw sperm condition using testes with intact epididymides obtained from a Siberian tiger (Panthera tigris altaica ) after castration. The epididymis was chopped in Tyrode's albumin-lactate-pyruvate 1x and incubated at 5% CO2, 95% air for 10 min. The Percoll separation density gradient method was used for selective recovery of motile spermatozoa after sperm collection using a cell strainer. The spermatozoa were diluted with modified Norwegian extender supplemented with 20 mM trehalose (extender 1) and subsequent extender 2 (extender 1 with 10% glycerol) and frozen using LN2 vapor. After thawing at 37℃ for 25 s, Isolate® solution was used for more effective recovery of live sperm. Sperm motility (computerized assisted sperm analysis, CASA), viability (SYBR-14 and Propidium Iodide) and acrosome integrity (Pisum sativum agglutinin with FITC) were evaluated. The motility of tiger epididymal spermatozoa was 40.1 ± 2.0%, and progressively motile sperm comprised 32.7 ± 2.3%. Viability was 56.3 ± 1.6% and acrosome integrity was 62.3 ± 4.4%. Cryopreservation of tiger epididymal sperm using a modified Norwegian extender and density gradient method could be effective to obtain functional spermatozoa for future assisted reproductive practices in endangered species.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
Animals
Epididymal sperm collection
Sperm freezing and thawing
Sperm motility
Sperm viability
Acrosome integrity
Statistics
RESULTS
DISCUSSION
CONCLUSION
REFERENCES

키워드

CASA cryopreservation epididymal sperm Siberian tiger

저자

  • Saddah Ibrahim [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University, Iksan 54596, Korea, Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum 204, Sudan ]
  • Nabeel Abdelbagi Hamad Talha [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University, Iksan 54596, Korea, Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum 204, Sudan ]
  • Jeongho Kim [ Cheongju Zoo, Cheongju 28311, Korea ]
  • Yubeol Jeon [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University, Iksan 54596, Korea ]
  • Iljeoung Yu [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University, Iksan 54596, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 37 No. 2

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.