Earticle

초록

영어

The aim of this study was to investigate the effects of exercise and diet on mental status, insulin signaling pathway, serotonin synthesis, and microbiome in high-fat-induced obesity mice. Before the start of this experiment, obesity groups made obese mice by administering a high-fat diet containing 60% fat for 12 weeks. In the obesity with exercise group, after a high-fat diet for 12 weeks, exercise was performed with high-fat diet for 8 weeks. In the obesity with diet group, a high-fat diet for 12 weeks followed by a normal diet for 8 weeks. Depression and anxiety were determined by open field test and elevated plus maze test. Immunohistochemistry for tryptophan hydroxylase (TPH) in the dorsal raphe, western blot analysis for phosphorylated protein kinase B (p-ATK), total AKT (t-AKT), phosphorylated phosphoinositide 3-kinase (p-PI3K), and total PI3K (t-PI3K) in the hippocampus were performed. Analysis of microbiome was also conducted. Obesity-induced depression and anx-iety status, suppressed ratio of p-AKT/t-AKT and p-PI3K/t-PI3K, and in-hibited TPH synthesis. Exercise and diet improved depression and anxi-ety status, activated p-AKT/t-AKT and p-PI3K/t-PI3K, and increased TPH synthesis. Exercise and diet improved depression and anxiety sta-tus by increasing the insulin signaling pathway and promoting serotonin production. These effects of exercise and diet were almost similar. In addition, exercise and diet regulated the composition of gut microbiota.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experiment animals and treatments
Treadmill exercise protocol
Open field test
Elevated plus maze test
Tissue preparation
Western blotting for insulin signaling
Immunohistochemistry for tryptophan hydroxylase in dorsal raphe
Collection of fecal samples, and sequencing and metagenomic analysis
Data analysis
RESULTS
The effects of exercise and diet on depression and anxiety
The effects of exercise and diet on insulin signaling molecules in the hippocampus
The effects of exercise and diet on microbiome in the gut microbiota
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Sang-Seo Park [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Tae-Woon Kim [ Department of Human Health Care, Gyeongsang National University, Jinju, Korea ]
• Bo-Kyun Kim [ Department of Emergency Technology, College of Health Science, Gachon University, Incheon, Korea ]
• Seong-Hyun Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ]
• Jong-Suk Park [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ]
• Mal-Soon Shi [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613