Earticle

초록

영어

The ascomycete fungus Cordyceps militaris infects lepidopteran insect pupae, forming characteristic fruiting bodies called “Dong Chung Ha Cho” in Korean. They have been used as medicines owing to their anti-allergic, anti-inflammatory, and immune-enhancing effects. This fungus can be grown on the geminated soybeans Rhynchosia nulubilis, which also contains several novel isoflavones. We performed a comparative transcriptome analysis to determine core gene sets or pathways contributing to biologically active products such as isoflavone. Initially, we sequenced 2-week-old fungal cultures on different soybean agar media, where different amounts of water agar were implemented to show different surface topology. We selected 830 upregulated and 188 downregulated genes by comparing linear models of the samples (two-fold change threshold). Gene ontology analysis identified that the “IMP biosynthesis” term was significantly found in the upregulated gene sets. The pathway is involved in the synthesis of cordycepin, the reference chemical for C. militaris. This finding in the transcriptome data is consistent with the previous observation: increased cordycepin concentrations in the C. militaris cultured on germinated soybean.

목차

ABSTRACT
Introduction
Materials and Methods
Fungal isolates and Fungal culture on thegerminated soybeans and its sampling
Extraction of RNA and RNA-Sequencing
Read filtering, mapping, and expression quantification
Gene ontology
Real-time quantitative reverse transcriptionPCR (qRT-PCR)
Results and Discussion
Growth on different germinated soybeans agar
Overall similarity between samples
Differential gene expression
Gene ontology and pathway analysis
REFERENCES

키워드

저자

• Chang-Hyuk Yoo [ Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University ]
• Jaehyuk Choi [ Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635