Earticle

초록

영어

We reported that application of ethanol with lipopolysaccharide (LPS) and carbon tetrachloride (CCl4) enhanced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level. In the current experi-ment, the protective effect of treadmill running on liver injury caused by ethanol with LPS and CCl4 in mice was studied. Liver injury severity was determined by measuring ALT and AST level in the blood. Terminal de-oxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for caspase-3, and Western blotting for Bcl-2-associated X protein (Bax) and B-cell lymphoma 2 (Bcl-2) were performed to indicate hepatocyte apoptosis. In addition, to understand the mechanism, 5’-adenosine monophosphate-activated protein kinase (AMPK) phosphorylation was studied by Western blotting. Treadmill ex-ercise ameliorated ethanol with LPS and CCl4-mediated elevation of ALT and AST level. Treadmill exercise suppressed ethanol with LPS and CCl4-mediated elevation of the TUNEL-positive cell number and cleaved caspase-3 expression. Treadmill exercise suppressed ethanol with LPS and CCl4-mediated elevation of Bax expression and increased Bcl-2 ex-pression suppressed by application of ethanol with LPS and CCl4. Tread-mill exercise enhanced AMPK phosphorylation which was suppressed by application of ethanol with LPS and CCl4. Treadmill exercise has the effect of reducing liver damage caused by alcohol and or drug addiction.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals and treatments
ALT and AST measurement in the blood
TUNEL staining
Caspase-3 immunohistochemistry
Bax, Bcl-2, AMPK Western blotting
Data analysis
RESULTS
ALT and AST level
TUNEL-positive cell number
Cleaved caspase-3 expression
Bax and Bcl-2 expressions
AMPK phosphorylation
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Sang-Hoon Kim [ Department of Sport & Health Sciences, College of Art & Culture, Sangmyung University, Seoul, Korea, Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jun-Jang Jin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Lakkyong Hwang [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Hye-Sun Yoon [ Department of Pediatrics, Eulji Hospital, Eulji University School of Medicine, Seoul, Korea ]
• Seung-Soo Baek [ Department of Sport & Health Sciences, College of Art & Culture, Sangmyung University, Seoul, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613