Earticle

초록

영어

We examined whether exercise is associated with hippocampus-medi-ated improvement in insulin signaling and cell differentiation in the triple transgenic mouse model of Alzheimer disease (3xTg AD) murine model following exposure to 40-Hz light flickering and exercise. We subjected 12-month-old 3xTg AD mice to exercise and 40-Hz light flickering for 3 months. The exercise session was proceeded for 12 consecutive weeks with gradual increase of intensity. To investigate insulin signaling proteins, western blot was conducted to detect the ratio of phosphory-lated insulin receptor β (p-IRβ)/total IRβ (t-IRβ), phosphorylated insulin receptor substrate 1 (p-IRS-1)/total IRS-1 (t-IRS-1), phosphorylated phosphatidylinositide-3-kinase (p-PI3K)/total PI3K (t-PI3K), phosphory-lated 3-phosphoinositide dependent protein kinase-1 (p-PDK1)/total PDK-1 (t-PDK1), phosphorylated protein kinase B (p-Akt)/total-Akt (t-Akt), and phosphorylated glycogen synthase kinase 3 beta (p-GSK3β)/total GSK3β (t-GSK3β). Doublecortin immunohistochemistry was per-formed for assessing cell differentiation in the hippocampus. Treatments exerted a positive effect. The combination of exercise and 40-Hz light flickering exposure was the most effective treatment enhancing insulin signaling. Increased ratio of p-IRβ/t-IRβ, p-IRS-1/t-IRS-1, p-PI3K/t-PI3K, p-PDK1/t-PDK1, p-Akt/t-Akt, and p-GSK3β/t-GSK3β and enhanced cell differentiation were observed in the 3xTg AD with exercise under 40-Hz light flickering group. Our results indicate that exercise under 40-Hz light flickering most potently improved insulin signaling, thereby promoted cell differentiation.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals
Exercise protocol and 40-Hz light flickering exposure time
Preparation of tissue
Immunohistochemistry
Western blotting
Statistical analysis
RESULTS
Effect of exercise with 40-Hz light flicker on the number of DCX-positive cells in hippocampal dentate gyrus
Effect of exercise with 40-Hz light flicker on the ratio of p-IRβ/t-IRβ and p-IRS-1/t-IRS-1 in hippocampus
Effect of exercise with 40-Hz light flicker on the ratio of p-PI3K/t-PI3K and p-PDK1/t-PDK1 in hippocampus
Effect of exercise with 40-Hz light flicker on the ratio of p-Akt/t-Akt and p-GSK3β/t-GSK3β in hippocampus
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Seong-Hyun Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, Division of Global Sport Studies, College of Culture and Sports, Korea University, Sejong, Korea ]
• Sang-Seo Park [ Department of Physiology, College of Medicine, Kyung Hee University, School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE, USA ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Tae-Woon Kim [ Department of Human Health Care, Gyeongsang National University, Jinju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613