Earticle

초록

영어

Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.

목차

Abstract
1. Introduction
2. Results and Discussion
2.1. Protective Effect of (-)-Loliode against UVB-Induced HaCaT Cell Damage
2.2. Protective Effect of (-)-Loliode against UVB-induced HDF Cell Damage
2.3. Protective Effect of (-)-Loliode against UVB-induced Zebrafish Damage
3. Materials and Methods
3.1. Chemical and Regents
3.2. Preparation of (-)-Loliode from S. horneri
3.3. In Vitro in HaCaT cells
3.4. In Vitro in HDF cells
3.5. In Vivo Assay
3.6. Statistical Analysis
4. Conclusions
References

키워드

저자

• LeiWang [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• Hyun-Soo Kim [ National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101gil, Janghang-eup, Seocheon 33662, Korea ]
• Jun-Geon Je [ Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea ]
• Xiaoting Fu [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• Caoxing Huang [ Co-Innovation Center for Efficient Processing and Utilization of Forest Products, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China ]
• Ginnae Ahn [ Department of Marine Bio Food Science, Chonnam National University, Yeosu 59626, Korea ]
• Jae-Young Oh [ Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea ]
• K. K. Asanka Sanjeewa [ Department of Biosystem Technology, Faculty of Technology, University of Sri Jayewardenepura, Pitipana, Homagama 10206, Sri Lanka ]
• Jiachao Xu [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• Xin Gao [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• In-Kyu Yeo [ Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea ] Corresponding Author
• You-Jin Jeon [ Department of Marine Life Sciences, Jeju National University, Marine Science Institute, Jeju National University, Jeju 63333, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551