Earticle

초록

영어

In the present study, the in vitro and in vivo anti-inflammatory effects of the sulfated polysaccharides isolated from Sargassum fulvellum (SFPS) were evaluated in lipopolysaccharide (LPS)- stimulated RAW 264.7 macrophages and zebrafish. The results indicated that SFPS improved the viability of LPS-stimulated RAW 264.7 macrophages from 80.02 to 86.80, 90.09, and 94.62% at the concentration of 25, 50, and 100 μg/mL, respectively. Also, SFPS remarkably and concentrationdependently decreased the production levels of inflammatory molecules including nitric oxide (NO), tumor necrosis factor-alpha, prostaglandin E2, interleukin-1 beta, and interleukin-6 in LPStreated RAW 264.7 macrophages. In addition, SFPS significantly inhibited the expression levels of cyclooxygenase-2 and inducible nitric oxide synthase in LPS-treated RAW 264.7 macrophages. Furthermore, the in vivo test results indicated that SFPS improved the survival rate of LPS-treated zebrafish from 53.33 to 56.67, 60.00, and 70.00% at the concentration of 25, 50, and 100 μg/mL, respectively. In addition, SFPS effectively reduced cell death, reactive oxygen species, and NO levels in LPS-stimulated zebrafish. Taken together, these results suggested that SFPS possesses strong in vitro and in vivo anti-inflammatory activities, and could be used as an ingredient to develop anti-inflammatory agents in the functional food and pharmaceutical industries.

목차

Abstract
1. Introduction
2. Results and Discussion
2.1. SFPS Suppresses Cytotoxicity and Inflammatory Molecules Production in LPS-Induced RAW 264.7 Macrophages
2.2. The Inhibitory Effect of SFPS against Pro-Inflammatory Cytokines, iNOS, and COX-2 Expression in LPS-Stimulated RAW 264.7 Macrophages
2.3. SFPS Improves LPS-Induced Inflammatory Response in Zebrafish
3. Materials and Methods
3.1. Reagents and Chemicals
3.2. Preparation of SFPS
3.3. Cell Culture
3.4. Measurement of NO Production and Cell Viability
3.5. Enzyme-Linked Immunosorbent Assay
3.6. Western Blot Analysis
3.7. Application of SFPS and LPS to Zebrafish
3.8. Measurement of Heartbeat, ROS Generation, NO Production, and Cell Death in Zebrafish
3.9. Statistical Analysis
4. Conclusions
References

키워드

저자

• LeiWang [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• Hye-Won Yang [ Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea ]
• Ginnae Ahn [ Department of Marine Bio Food Science, Chonnam National University, Yeosu 59626, Korea ]
• Xiaoting Fu [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• Jiachao Xu [ Jiachao Xu | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• Xin Gao [ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China ]
• You-Jin Jeon [ Department of Marine Life Sciences, Jeju National University, Marine Science Institute, Jeju National University, Jeju 63333, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551