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Molecular characterization, immune and xenobiotic responses of glutathione S-transferase omega 1 from the big-belly seahorse: Novel insights into antiviral defense

첫 페이지 보기
  • 발행기관
    제주대학교 해양과학연구소 바로가기
  • 간행물
    해양과학연구소 연구논문집 바로가기
  • 통권
    제45권 (2021.12)바로가기
  • 페이지
    pp.52-60
  • 저자
    H.M.V. Udayantha, D.S. Liyanage, Kishanthini Nadarajapillai, W.K.M. Omeka, Hyerim Yang, Taehyug Jeong, Jehee Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A406935

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원문정보

초록

영어
Glutathione S-transferases (GSTs) are important enzymes involved in phase II detoxification and function by conjugating with the thiol group of glutathione. In this study, we isolated an omega class GST from the big-belly seahorse (Hippocampus abdominalis; HaGSTO1) to study the putative xenobiotic responses and defense ability against viral and bacterial infections in this animal. The isolated HaGSTO1 gene, with a cording sequence of 720 bp, encodes a peptide of 239 amino acids. The predicted molecular mass and theoretical isoelectric point of HaGSTO1 was 27.47 kDa and 8.13, respectively. In-silico analysis of HaGSTO1 revealed a characteristic N-terminal thioredoxin-like domain and a C-terminal domain. Unlike other GSTs, the C-terminal of HaGSTO1 reached up to the N-terminal, and the N-terminal functional group was cysteine rather than tyrosine or serine, as observed in other GSTs. Phylogenetic analysis showed the evolutionary proximity of HaGSTO1 with other identified vertebrate and invertebrate GST orthologs. For the first time, we demonstrated the viral defense capability of HaGSTO1 against viral hemorrhagic septicemia virus (VHSV) infection. All six nucleoproteins of VHSV were significantly downregulated in HaGSTO1-overexpressing FHM cells at 24 h after infection compared with those in the control. Moreover, arsenic toxicity was significantly reduced in HaGSTO1-overexpressing FHM cells, and cell viability increased. Real-time polymerase chain reaction analysis showed that HaGSTO1 transcripts were highly expressed in the pouch and gill when compared with those in other tissues. Blood HaGSTO1 transcripts were significantly upregulated after Edwardsiella tarda, Streptococcus iniae, lipopolysaccharide, and polyinosinic: polycytidylic acid challenge experiments. Collectively, these findings suggest the involvement of HaGSTO1 in the host defense mechanism of seahorses.

목차

ABSTRACT
1. Introduction
2. Materials and methods
2.1. HaGSTO1 sequence identification and in-silico analysis
2.2. Seahorse acclimatization and tissue sampling
2.3. Immune challenge experiment
2.4. Total RNA extraction and cDNA synthesis
2.5. HaGSTO1 expression analysis
2.6. Cloning of HaGSTO1 into expression plasmids
2.7. Production and purification of recombinant HaGSTO1 (rHaGSTO1) fusion protein
2.8. Biochemical properties of rHaGSTO1
2.9. Cell viability in the presence of arsenic
2.10. Antiviral activity of HaGSTO1 in response to VHSV
3. Results and discussion
3.1. HaGSTO1 sequence characterization
3.2. Homology analysis of HaGSTO1
3.3. Analysis of the gene expression profile of HaGSTO1
3.4. Enzymatic activity of rHaGSTO1
3.5. Detoxification activity of HaGSTO1
3.6. Antiviral activity of HaGSTO1 against VHSV infection
4. Conclusion
References

키워드

Glutathione S-Transferases omega 1 Detoxification Viral hemorrhagic septicemia virus infection Antiviral response Immune response Seahorse

저자

  • H.M.V. Udayantha [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea ]
  • D.S. Liyanage [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea ]
  • Kishanthini Nadarajapillai [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea ]
  • W.K.M. Omeka [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea ]
  • Hyerim Yang [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea ]
  • Taehyug Jeong [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea ]
  • Jehee Lee [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    제주대학교 해양과학연구소 [Marine Science Institute Jeju National University]
  • 설립연도
    1968
  • 분야
    농수해양>수산학
  • 소개
    해양의 실체와 그 자원의 합리적 이용과 관리방법을 규명하기 위하여 해양물리, 화학, 생물, 지질, 공학등 여러 관련분야에 관한 다양한 연구를 수행하며, 환경의 보전과 보호를 위해서 수질, 대기, 상·하수도, 폐기물, 사회적 환경 등의 연구를 통하여 쾌적하고 청정한 환경을 보전하기 위한 다양한 연구를 수행하고, 또한 해양 및 환경분야의 우수한 인력양성, 국내외 학술교류의 증진, 학·산 협동체제의 확립 등을 통하여 해양과 환경에 관한 기초 및 응용적 학술연구를 종합적으로 수행함을 목적으로 한다.

간행물

  • 간행물명
    해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]
  • 간기
    폐간
  • pISSN
    1225-5734
  • 수록기간
    1977~2021
  • 십진분류
    KDC 454 DDC 551

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