Earticle

초록

영어

Paired box protein, PAX7, is a key molecule for the specification, maintenance and skeletal muscle regeneration of muscle satellite cells. In this study, we identified and characterized the cDNA and amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii ) via molecular cloning and sequence analysis. A. schlegelii PAX7 cDNA was comprised of 1,524 bp encoding 507 amino acids and multiple sequence alignment analysis of the translated amino acids showed that it contained three domains including paired DNA-binding domain, homeobox domain and OAR domain which were well conserved across various animal species investigated. Pairwise Sequence Alignment indicated that A. schlegelii PAX7 had the same amino acid sequences with that of yellowfin seabream (A. latus ) and 99.8% identity and similarity with that of gilt-head bream (Sparus aurata ). Molecular phylogenetic analysis confirmed that A. schlegelii PAX7 formed a monophyletic group with those of teleost and most closely related with those of the fish that belong to Sparidae family including A. latus and S. aurata . In the investigation of its tissue specific mRNA expression, the expression was specifically identified in skeletal muscle tissue and a weak expression was also shown in gonad tissue. The cultured cells derived from skeletal muscle tissues expressed PAX7 mRNA at early passage but the expression was not observed after several times of subculture.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Fish
cDNA Sequencing of PAX7 gene
Characterization of the deduced amino acid sequences
Primary explant culture
Reverse transcription-PCR (RT-PCR)
RESULTS AND DISCUSSION
REFERENCES

키워드

저자

• Jae Hoon Choi [ Department of Fisheries Biology, Pukyong National University, Busan 48513, Korea ]
• Dan Hee Han [ Department of Fisheries Biology, Pukyong National University, Busan 48513, Korea ]
• Seung Pyo Gong [ Department of Fisheries Biology, Pukyong National University, Department of Marine-Biomaterials and Aquaculture, Pukyong National University, Busan 48513, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636