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Traf4 is required for tight junction complex during mouse blastocyst formation
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Journal of Animal Reproduction and Biotechnology 바로가기
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- 통권
- Volume. 36 No. 4 (2021.12)바로가기
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- 페이지
- pp.307-313
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A406447
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원문정보
초록
- 영어
- Traf4 (Tumor necrosis factor Receptor Associated Factor 4) is a member of the tumor necrosis factor receptor (TNFR) - associated factors (TRAFs) family. TRAF4 is overexpressed in tumor cells such as breast cancer and associated with cytoskeleton and membrane fraction. Interestingly, TRAF4 was localized with tight junctions (TJs) proteins including OCLN and TJP1 in mammary epithelial cells. However, the expression patterns and biological function of Traf4 were not examined in preimplantation mouse embryos although Traf4-deficient mouse showed embryonic lethality or various dramatic malformation. In this study, we examined the temporal and spatial expression patterns of mouse Traf4 during preimplantation development by qRT-PCR and immunostaining, and its biological function by using siRNA injection. We found upregulation of Traf4 from the 8-cell stage onwards and apical region of cell – cell contact sites at morula and blastocyst embryos. Moreover, Traf4 knockdown led to defective TJs without alteration of genes associated with TJ assembly but elevated p21 expression at the KD morula. Taken together, Traf4 is required for TJs assembly and cell proliferation during morula to blastocyst transition.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Embryo collection and microinjection
Real-time quantitative PCR
Immunocytochemistry
FITC-dextran uptake assay
Statistical analysis
RESULTS AND DISCUSSION
Expression of TRAF4 in mouse preimplantation embryos
Effects of Traf4 KD on preimplantation embryo development
Effects of Traf4 KD on the gene expression associated with TJs and the cell cycle
CONCLUSION
REFERENCES
키워드
blastocyst preimplantation p21 tight junction Traf4저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Journal of Animal Reproduction and Biotechnology
- 간기
- 계간
- pISSN
- 2671-4639
- eISSN
- 2671-4663
- 수록기간
- 2019~2025
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 36 No. 4
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