Earticle

초록

영어

Organ transplantation is currently the most fundamental treatment for organ failure, but there is a shortage of organ supply compared to those in need. Regenerative medicine has recently developed a decellularization technique that overcomes the limitations of conventional organ transplantation and attempts to reconstruct damaged tissues or organs to their normal state. Several decellularization methods have been suggested. In this experiment, the decellularization methods were used to find effective decellularization methods for humanlike porcine placenta. The optimal conditions for decellular support are low DNA content and high glycos amino glycans (GAGs) and collagen content. In order to satisfy this condition, SDS and Triton X-100 and SDS + Triton X-100 were used as the detergent used for decellularization in this experiment. The contents were compared according to the decellularization time (0, 12, 24, 48 and 72 hours), and the concentrations of SDS (0.2, 0.5, 0.7 and 1.0%) were mixed in 1.0% Triton X-100 to analyze the contents. When decellularized using SDS and Triton X-100, respectively, it was confirmed that the contents of DNA and GAGs were opposite to each other. And decellularization treatment for 24 hours at 0.5% SDS was able to obtain an effective decellular support. If decellularization studies of various detergents can be obtained an effective decellular support, and furthermore, cell culture experiments can confirm the effect on the cells.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Porcine placenta preparation
Experimental design
Decellularization of porcine placenta
Freeze-drying of decellularized porcine placenta
Histological analysis of decellularized porcine placenta
Analysis of extracellular matrix (ECM) components in decellularized porcine placenta
Statistical analysis
RESULTS
Changes in placenta shape, total DNA and DNAstaining with different decellularization times
GAGs and collagen contents of porcine placenta according to decellularization detergent
DNA content and GAGs content of porcine placenta decellularized by mixing 1.0% Triton X-100 in each concentration of SDS
DISCUSSION
REFERENCES

키워드

저자

• Ji Hyung Son [ Department of Animal Resources, Daegu University, Maria S IVF Center, Seoul 02098, Korea ]
• Dae-Jung Kim [ Jeju Fisheries Research Institute, National Fisheries Research and Development Institute, Jeju 63068, Korea ]
• Dong-Mok Lee [ Biomedical Manufacturing Technology Center, Korea Institute of Industrial Technology, Yeoncheon 38822, Korea ] Corresponding Author
• Byoung Boo Seo [ Department of Animal Resources, Daegu University, Institute of Natural Sciences, Daegu University, Gyeongsan 38453, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636