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Original Article

Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 36 No. 4 (2021.12)바로가기
  • 페이지
    pp.239-246
  • 저자
    So-Yeon Jo, Yong Hwangbo, Sang-Hee Lee, Hee-Tae Cheong, Dong-Ku Kim, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A406439

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원문정보

초록

영어
The immunological sperm separation method is economical compared to the existing sorting method, and it is promising for the development of new technologies by reducing sperm damage. Wholemom (WM) is a sex-regulating protein that comprises on immunoglobulin G coupled with magnetic nanoparticles (MNPs) that responds to surface proteins derived from the Y chromosome in cattle. Y sperms are restricted in motility as the WM aggregates them, and the magnet could separate the non-aggregated cells. This study was conducted to investigate the effect of WM treatment on the characteristics of bull sperm. After treating sperm with WM and incubation for 6 h, the motility parameters including total motility, progressive motility, velocity average path, velocity straight line, amplitude of lateral head displacement, and linearity were significantly higher in the WM treatment group than in the control group (p < 0.05). Sperm viability and acrosome reaction rates were similar in both groups during each incubation period (p > 0.05). In conclusion, the immunological sperm sexing procedure using a monoclonal antibody conjugated with MNPs did not affect the characteristics of bull sperm. This study suggests that compared to other techniques, the immunological method for sperm sexing could classify sperm quickly and efficiently without the use of expensive equipment.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
Preparation of sexed sperm
Computer-assisted sperm analysis (CASA)
Fluorescent staining and flow cytometry analysis
Statistical analysis
RESULTS
Effect of WM treatment on the agglutination of bull sperm
Effect of WM treatment on the characteristics of bull sperm
DISCUSSION
CONCLUSION
REFERENCES

키워드

immunological method sperm characteristics sperm sexing Y specific antibody

저자

  • So-Yeon Jo [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
  • Yong Hwangbo [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
  • Sang-Hee Lee [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Dong-Ku Kim [ Nuriscience Inc, Seoul 05053, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2026
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 36 No. 4

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