Earticle

초록

영어

Diabetic mellitus (DM) is a carbohydrate metabolic disorder that involves high blood sugar because insulin works abnormally. Type 2 diabetes accounts for most of them. However, diabetes treatments such as GLP-1 and DPP-4 inhibitors commonly caused side effects including gastrointestinal disorders. Grifola frondosa (G. frondosa) revealed various pharmacological effects in recent studies. It has a variety of anti-cancer polysaccharides through host-mediated mechanisms. D-fraction in G. frondosa has apoptotic effects, promoting myeloid cell proliferation and differentiation into granulocytes-macrophages. It has also been shown to reduce the survival rate of breast cancer cells. Though, no further study has been conducted on the specific effects of G. frondosa in the db/db mouse. Therefore, we would like to research the blood glucose improving effect of G. frondosa, a natural material, in type 2 diabetes model mouse, in this study. G. frondosa was administered to the disease model mouse (BKS.Cg-+Leprdb/+Leprdb/OlaHsd) for 8 weeks to monitor weight and blood glucose changes every week. And we evaluated anti-diabetes effects by checking biomarker changes shown through blood. Experiment did not show statistically significant weight differences, but control groups showed significantly higher weight gain than G. frondosa administered groups. We collected blood from the tail veins of the db/db mouse each week. As a result, the lowest blood sugar level was shown in the 500 mg/ kg group of G. frondosa. Glucose in the blood was examined with HBA1c, and 7.8% was shown in the 500 mg/kg administration group, lower than in other groups. These results suggest the potential improvements of diabetes in G. frondosa.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animal model and experimental design
Autopsy and blood collection
Blood biochemical test
Measurement of HbA1c
Plasma MDA
Real-Time polymerase chain reaction (PCR)
Statistical analysis
RESULTS
Body weight
Effects of G. frondosa on blood sugar level
Biochemical test of lipid
Glucose level test with hemoglobin A1c
Lipid peroxidation (MDA) assay
Analysis of mRNA expression levels of IR, IRS1, Pl3k, Akt
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Seong-Bo Yun [ Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Korea ]
• Dae-Young Kim [ Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636