Earticle

초록

영어

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA3 on the growth of plants germinated from zygotic embryos through GA3 treatment, the best growth was shown in 0.5 mg/l GA3 treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

목차

Abstract
Introduction
Materials and Methods
Plant materials and in vitro germination
Shoot elongation of germinated plants
In vitro rooting and propagation
Acclimatization of in vitro plants
Statistical analysis
Results and Discussion
Germination of in vitro zygotic embryos
Shoot elongation of germinated plants
In vitro growth according to the cultivation period
Acclimatization of in vitro plants
Acknowledgements
References

키워드

저자

• Eun ji Choi [ Plant Conservation Division, Korea National Arboretum of the Korea Forest Service, Pochen 11186, Republic of Korea ]
• Seong Hyeon Yong [ Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Yu Won Seol [ Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Dong Jin Park [ Department of Seed and Seedling Management, National Forest Seed and Cultivar Center, Chungju 27495, Republic of Korea ]
• Kwan Been Park [ Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Do Hyun Kim [ Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Eon Ju Jin [ Forest Biomaterials Research Center, National Institute of Forest Science, Jinju 52828, Republic of Korea ]
• Myung Suk Choi [ Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634