Kee-Hwa Bae, I-Seul Yun, Ji-Sun Jung, Chan-Beom Kim, Hye-Won Kim, Yong-Sik Hong, Min-Kyeong Oak, Hak-Koo Kim, Ju-Hui Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A405837
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※ 학술발표대회집, 워크숍 자료집 중 4페이지 이내 논문은 '요약'만 제공되는 경우가 있으니, 구매 전에 간행물명, 페이지 수 확인 부탁 드립니다.
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원문정보
초록
영어
Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.
목차
Abstract Introduction Materials and Methods Results and Discussion Acknowledgements References
Kee-Hwa Bae [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Corresponding Author
I-Seul Yun [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Ji-Sun Jung [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Chan-Beom Kim [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Hye-Won Kim [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Yong-Sik Hong [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Min-Kyeong Oak [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Hak-Koo Kim [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]
Ju-Hui Lee [ Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum, Sejong 30106, Republic of Korea ]