Earticle

초록

영어

The effect of combined approach of exercise training and bone marrow stromal cell (BMSC) engraftment on activation of brain-derived neuro-trophic factor (BDNF)-extracellular signal-regulated kinase 1 and 2 (ERK1/2)-cyclic adenosine monophosphate response element-binding protein (CREB) signaling pathway after sciatic nerve injury (SNI) was investigated. Sixty male Sprague-Dawley rats divided into the normal control, nonexercise (NEX), exercise training (EX), BMSC transplanta-tion (TP), and exercise training+BMSC transplantation (EX+TP) groups 4 weeks after SCI. Exercise training was carried out on the treadmill de-vice at 5–10 m/min for 20 min for 4 weeks. Single dose of 5×106 harvest-ed BMSC was injected into the injury area of the injured sciatic nerve. In order to evaluate induction levels of BDNF-ERK1/2-CREB signaling molecules in the whole cell and nuclear cell lysates of the injured sciat-ic nerve, we applied Western blot analysis. BDNF was significantly in-creased only in EX+TP compared to NEX, EX, and TP groups. Phospho-inositide-dependent kinase-1 was more increased in EX, TP, and EX+TP groups than NEX group, but EX+TP group showed the most upregula-tion of phosphorylated protein kinase B compared to other groups. In addition, in the whole cell lysate, phosphorylated ERK1/2, but not acti-vating transcription factor-3 (ATF-3) and phosphorylated CREB, was sig-nificantly increased in TP and EX+TP groups. In the nuclear cell lysate, ATF-3 and phosphorylated CREB were strongly activated in EX+TP group compared to EX group. Regular exercise training combined with BMSC engraftment would seem to be more effective in controlling activation of regeneration-related signaling pathway after SNI.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
Sciatic nerve injury
BMSC culture and transplantation
Treadmill exercise
Western blot analysis
Statistical analysis
RESULTS
Activation of BDNF-Akt signaling pathway 28 days after SNI
Activation of ERK1/2-CREB pathway in whole cell lysate28 days after SNI
Activation of ATF-3 and p-CREB in the nucleus 28 days after SNI
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Yeong-Hyun Cho [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Hyuk Sakong [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Young-Pyo Kim [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613