Earticle

초록

영어

The purpose of this study was to investigate whether combination of low-intensity exercise with bone marrow stromal cell (BMSC) trans-plantation could regulate protein kinas B (Akt)- mammalian target of rapamycin (mTOR) and Wnt3a-β-catenin signaling pathways for pre-vention of soleus muscle atrophy after sciatic nerve injury (SNI). The experimental rats divided into 5 groups (n=10): normal control group, SNI+sedentary group (SED), SNI+low-intensity treadmill exercise group (TEX), SNI+BMSC transplantation group (BMSC), SNI+TEX+BMSC transplantation group (TEX+BMSC). Sciatic nerve crush injury was ap-plied into the middle of thigh twice for 1 min and 30 sec at interval. Low-intensity treadmill exercise was comprised of walking at a speed of 4 to 8 m/min for 30 min once a day. cultured BMSC at a density of 5×106 in 50-μL phosphate-buffered saline was injected into the distal portion of the injured sciatic nerves. TEX+BMSC group dramatically up-regulated expression levels of growth-associated protein-43 in the in-jured sciatic nerve at 2 weeks postinjury. Also, although Akt and mTOR signaling pathway significantly increased in TEX and BMSC groups than SED group, TEX+BMSC group showed more potent increment on this signaling in soleus muscle after SNI. Lastly, Wnt3a and the nuclear translocation of β-catenin and nuclear factor-kappa B in soleus were increased by SNI, but TEX+BMSC group significantly downregulated activity of this signaling pathway in the nuclear cell lysate of soleus muscle. Present findings provide new information that combination of low-intensity treadmill exercise might be effective therapeutic ap-proach on restriction of soleus muscle atrophy after peripheral nerve injury.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
BMSC culture
SNI and BMSC transplantation
Treadmill exercise
Western blot analysis
Immunofluorescence staining
Statistical analysis
RESULTS
Expression levels of GAP-43 in the injured sciatic nerve
Activation of Akt/mTOR signaling pathway in the soleus after SNI
Activation of canonical Wnt3a-β-catenin signaling pathway in soleus after SNI
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Yeong-Hyun Cho [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613