Earticle

현재 위치 Home

Original Article

DNA recombinase Rad51 is regulated with UVinduced DNA damage and the DNA mismatch repair inhibitor CdCl2 in HC11 cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 36 No. 3 (2021.09)바로가기
  • 페이지
    pp.121-128
  • 저자
    Hyeong-Ju You, Ga-Yeon Kim, Seung-Yeon Kim, Man-Jong Kang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A401384

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

원문정보

초록

영어
Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl2. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 μm of CdCl2, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl2 untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl2 in HC11 cells decreased the mRNA expression of Rad51.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RT-PCR
Cell culture and transfection
UV irradiation
Immunocytochemistry
CdCl2 treatment
RT-qPCR analysis
Statistical analysis
RESULTS
The mRNA expression of Dmc1 and Rad51 in the HC11 cell and testes
The protein expression of Dmc1 and Rad51 in the HC11 cells treated with UV
The mRNA expression of DNA MMR related gene and Rad51 in the HCll cells treated CdCl2
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

cadmium chloride homologous recombination knock-in mismatch repair Rad51 recombinase

저자

  • Hyeong-Ju You [ Department of Animal Science, Chonnam National University, Gwangju 61186, Korea ]
  • Ga-Yeon Kim [ Department of Animal Science, Chonnam National University, Gwangju 61186, Korea ]
  • Seung-Yeon Kim [ Department of Animal Science, Chonnam National University, Gwangju 61186, Korea ]
  • Man-Jong Kang [ Department of Animal Science, Chonnam National University, Gwangju 61186, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2026
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 36 No. 3

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.